Normal Clinical Values in Adult Horses (2025 Update) – Equine Vital Signs, Bloodwork & Biochemistry Explained

🐴 Normal Clinical Values in Adult Horses

Physiological Parameters of Horse

ParameterNormal RangeNotes
Rectal Temperature37.2 – 38.6 °CFever > 38.6 °C; hypothermia < 36.5 °C. ❄️ Low readings in cold environments may not indicate true hypothermia.
Heart Rate28 – 44 bpmUp to 60 bpm in neonates; ↑ in pain, colic, or stress. Fit athletes may have resting HR 24–28 bpm. HR >60 bpm at rest is abnormal.
Respiratory Rate8 – 16 breaths/min↑ in pain, fever, or respiratory disease. ⚠️ Up to 80/min post-exercise is normal; wait 30 min for accurate resting value.
Oestrous Cycle18 – 24 days (avg: 21 d)Seasonally polyestrous (spring/summer); influenced by daylight length.
Gestation Period320 – 360 days (avg: 340 d)Foaling >360 days still within normal; consider fetal viability, not calendar alone.

Hematological Parameters of Horse

ParameterNormal RangeNotes
Hemoglobin11.2 – 17.2 g/dLMay increase with dehydration or high altitude training.
Packed Cell Volume (PCV/HCT)30 – 46 %↑ in dehydration; 💧 PCV >50% + tacky MM = severe dehydration. Repeat after rehydration.
RBC Count6.2 – 10.2 million/µLHigher at altitude or in trained horses (splenic contraction effect).
WBC Count5,500 – 12,500/µL⭐️ Stress leukogram = ↑ neutrophils, ↓ lymphocytes. Confirm with clinical context and smear.
Platelet Count100,000 – 350,000/µL🔍 Smear review essential: clumping may falsely lower automated count.
Normal Clinical Values in Adult Horses (2025 Update) – Equine Vital Signs, Bloodwork & Biochemistry Explained

Liver & Muscle Biomarkers of Horse

ParameterNormal RangeNotes
AST (SGOT)100 – 600 U/L⏳ ↑ in liver, muscle, or hemolysis. Persistent elevation (7–10 days) = chronic injury.
ALT26 – 34 U/LNot liver-specific in horses. Less clinically relevant than in small animals.
ALP68 – 387 U/L↑ in young horses (bone growth); moderate ↑ in cholestasis or liver disease.
GGT0 – 7 U/L⚠️ Most liver-specific enzyme in horses. Elevates slowly; peaks in 5–7 days.
LDH240 – 1,300 U/LNonspecific; ↑ in muscle injury, liver disease, or hemolysis. Interpret with AST/CK.
Creatine Kinase (CK)100 – 350 U/L💥 CK >10,000 U/L = acute exertional rhabdomyolysis. Peaks early (24–48 hrs).

Kidney Function Parameters of Horse

ParameterNormal RangeNotes
BUN (Urea)10 – 25 mg/dLMild ↑ in dehydration or high-protein intake; not always renal-specific.
Creatinine0.8 – 2.2 mg/dL⚠️ Draft horses may reach 2.5 mg/dL; rising trend more important than absolute number.

General Biochemistry of Horse

ParameterNormal RangeNotes
Total Protein5.8 – 8.0 g/dL↑ in dehydration; ↓ in protein loss or poor intake
Albumin1.9 – 3.2 g/dLLiver-synthesized; ↓ in liver disease or protein loss
Globulin2.8 – 5.1 g/dL↑ in chronic inflammation/infection
Triglycerides50 – 110 mg/dL↑ in negative energy balance (e.g., miniature horses)
Cholesterol50 – 110 mg/dLMuch lower than in carnivores; ↑ not often significant
Glucose56 – 88 mg/dLMild ↑ with stress; ↓ in sepsis or anorexia

Electrolytes Count of Horse

ElectrolyteNormal RangeNotes
Calcium (total)10.4 – 12.9 mg/dL↑ with dehydration or hyperparathyroidism
Phosphorus4.4 – 6.3 mg/dL↓ in adult horses with poor nutrition
Magnesium1.5 – 2.5 mg/dLVital for neuromuscular function
Sodium132 – 146 mEq/LMatches corrected range for adult horses
Potassium3.5 – 5.8 mEq/LHypokalemia in anorexia, alkalosis; ↑ in renal failure
Chloride97 – 109 mEq/L↓ in metabolic alkalosis or excessive sweating

Other Key Tips

  • Hemolysis Artifact: Pink plasma → falsely ↑ K⁺, AST, LDH; ↓ Ca²⁺. Reject sample if hemolyzed.
  • False-Normal TGs: In starving minis, TG may be falsely normal early. Lipemia = warning sign.
  • Temp-HR Relationship: Every 1°C increase = ~10 bpm ↑ HR. Fever without tachycardia suggests toxemia.
  • Creatinine Trends: Rising from 0.9 → 1.8 mg/dL = clinically significant. Consider imaging or renal clearance testing.

Final Clinical Recommendations

Never interpret labs in isolation — always correlate with physical exam.

Breed-Specific Norms Matter:

  • Drafts = higher CK/creatinine
  • Arabians = lower WBC
  • Miniatures = high TG risk

Use Dynamic Testing When Indicated:

  • Bile acids for liver function
  • Fractional excretion for renal evaluation

Always Request Lab Reference Ranges:
Each lab uses different analyzers and standards — context is crucial.


“Context is king. No number is an island.”
Use reference values as a guide, not a diagnosis.


Discover the complete list of normal clinical values in adult horses – including temperature, heart rate, PCV, liver & kidney biomarkers, electrolytes, and more. Updated for 2025 with clear tables and practical vet notes.

THE RAJASTHAN EXPRESS